國立臺灣大學生態學與演化生物學研究所

Institute of Ecology and Evolutionary Biology
National Taiwan University

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Hurng-Yi Wang, Professor (王弘毅 教授)


Title: Professor

Education: Ph.D., National Taiwan Normal University, Taiwan
Interest: Population Genetics, Molecular Evolution, Genomics

E-mail: hurngyi@ntu.edu.tw
Lab:
Tel: +886-2-23123456 ext 66614
Fax:


Research Interests

(1) Molecular evolution of hepatitis B virus (HBV)

Hepatitis B virus (HBV) is one of the most common infectious agents in the world. According to world health organization (WHO), more than a third of the world’s population (2 billion people) has been infected with HBV. In Taiwan, 15-20% of the population are chronic HBV carriers which translate to 3.5-4 million people nation-wide.

We collaborate with Prof Chang, MH in Department of Pediatric, National Taiwan University Hospital, who has collected serum samples every six months from a cohort of HBV carriers for more than 17 years, beginning before the age of 10 years. For each patient, multiple full-length HBV sequences were recovered from three time points spanning the immunotolerant phase without alanine aminotransferase (ALT) elevation to the early immunoclearance phase with mild ALT elevation. We found that the interplay between viral replication and host immunity explains patterns of HBV dynamics within host during relative early stage of infection. That is, without immune selection, competition between peers increases the viral load and decreases nucleotide diversity; in contrast, host immunity accelerates viral evolution and decreases copy numbers but increases diversity (Wang et al. 2010). We are currently analyzing more individuals with different disease stages. Our observation may have important implications for studying long-term HBV adaptation and therapeutic design for chronic hepatitis infection.

(2) Quantitative trait loci (QTL) mapping of hepatitis viral susceptibility

It has been shown previously that different inbred mouse strains have different response to an engineered and replication-competent pAAV/HBV1.2 DNA. After hydrodynamical injection of pAAV/HBV1.2 DNA, the serum HBsAg level dropped faster in BALB/c mice than in C57BL/6 mice. As a result, 80% of C57BL/6 mice were still HBsAg-positive 35 days after injection, whereas none of BALB/c mice was HBsAg-positive after 28 days.

We hypothesize that genetic background of different inbred mice play a crucial role in the persistence of HBV. The ultimate goal of this study is to dissect the genetic component of HBV tolerance in mice and its possible connection with chronic HBV infection in human. To that end, we perform QTL mapping to delineate the genetic components that control viral susceptibility.

(3) Transcription regulation at incipient stage of speciation

How species forms is one of the most fascinating and mysterious question in biology. The Zimbabwe race of D. melanogaster vis-a-vis the cosmopolitan populations provides a good opportunity to study speciation at very early stage. Females from most Z lines (for Zimbabwe) do not mate with males from M lines (for melanogaster of the cosmopolitan type), whereas the reciprocal crosses experience much weaker or no isolation.

In this project, our main interest is how gene expression is regulated between different racial groups. To that end, we apply microarray and pyrosequencing technologies to study gene regulation at globally and individually. At whole genome level, we found most of genes are jointly regulated by cis- and trans-factors. In addition, cis-component increases with increase level of divergence (Wang et al 2008). We also study allelic specific expression using pyrosequencing technology. We demonstrate that gene expression is highly context-dependent, i.e. genetic background has strong influence on the expression of the gene.


Courses offered

EEB5045 族群遺傳學
EEB5035 演化生物學
EEB5087 分子演化

Publications

Google Scholar Research Gate

  1. Chaw SM, Tai JH, Chen SL, Hiseh CH, Chang SY, Yeh SH, Yang WS, Chen PJ, Wang HY*. The origin and underlying driving forces of the SARS-CoV-2 outbreak. Journal of Biomedical Science. In press (IF=5.203; Medicine, Research, and Experimental 20/136=14.7%)
  2. Hsieh CH, Huang CG*, Wu WJ, and Wang HY*. A rapid insect species identification system using mini‐barcode pyrosequencing. Pest management science 2019. 76 (4), 1222-1227. (IF: 3.255; Entomology 6/98= 6%)
  3. Wu LL, Peng WH, Wu H-L, Miaw SC, Yeh SH, Yang HC, Liao PH, Lin JS, Chen YR, Hong YT, Chen PJ, Wang HY*, and Chen DS*. Ly6C+ Monocytes and Kupffer Cells Orchestrate Liver Immune Responses Against Hepatitis B Virus in Mice. Hepatology, 2019. 69: 2364-2380. (IF: 14.971; Gastroenterology and hepatology 5/84=6%)
  4. Zhang Y, Li Y, Shen X, Zhu T, Tao Y, Li T, Li X, Wang D, Ma Q, Hu Z, Liu J, Zheng C, Ruan J, Cai J, Wu CI, Lu X*, and Wang HY*. “Genetic load and potential mutational meltdown in cancer cell populations” Molecular Biology and Evolution, 2019. 36: 541-552. (IF: 14.797; Genetics and heredity 4/173=2%)
  5. Wen H, Wang HY, He H and Wu CI*. “On the low reproducibility of cancer studies.” National Science Review, 2018, in press. (IF: 13.22; Multidisciplinary Sciences 3/69=4%)
  6. Wang HY, Chen Y, Tong D, Ling S, Hu Z, Tao Y, Lu X and Wu CI*. “Is the evolution in tumors Darwinian or non-Darwinian?” National Science Review. 2018, 5: 15-17. (IF: 13.22; Multidisciplinary Sciences 3/69=4%)
  7. Lin YY, Hsieh CH, Chen JH, Lu X, Kao JH, Chen PJ, Chen DS, Wang HY*. “De novo assembly of highly polymorphic metagenomic data using in situ generated reference sequences and a novel BLAST-based assembly pipeline.” BMC Bioinformatics. 2017, 18(1):223. (IF: 2.448; Mathematical and computational biology 14/59=23%)
  8. Wu CI*, Wang HY, Ling S, Lu X. “The Ecology and Evolution of Cancer: The Ultra-Microevolutionary Process.” Annual Review Genetics. 2016, 50:347-369. (IF: 9.589; Genetics and heredity 9/171=5%)
  9. Lin YY, Liu C, Chien WH, Wu LL, Tao Y, Wu D, Lu X, Hsieh CH, Chen PJ, Wang HY*, Kao JH and Chen DS*. “New insights into the evolutionary rate of hepatitis B virus at different biological scales.” Journal of Virology. 2015, 89(7): 3512-22. (IF: 4.368; Virology 8/35=22%)
  10. Chou HH, Chien WH, Wu LL, Cheng CH, Chung CH, Horng JH, Ni YH, Tseng HT, Wu D, Lu X, Wang HY*, Chen PJ and Chen DS*. “Age-related immune clearance of hepatitis B virus infection requires the establishment of gut microbiota.” Proc Natl Acad Sci USA. 2015, 112(7): 2175-80. (IF: 9.504; Multidisciplinary Sciences 5/64=7%)
  11. Tseng SP, Li SH, Hsieh CH, Wang HY* and Lin SM*. “Influence of gene flow on divergence dating – implications for the speciation history of Takydromus grass lizards.” Molecular Ecology. 2014, 23(19): 4770-84. (IF:6.131; Ecology 10/158=6%)
  12. Hsieh CH, Ko CC, Chung CH and Wang HY*. “Multilocus approach to clarify species status and the divergence history of the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex.” Molecular Phylogenetic and Evolution. 2014, 76: 172-80. (IF: 4.412 Genetics and heredity 34/171=19%)
  13. Chiou HY, Hsieh CH, Jeng CR, Chan FT, Wang HY* and Pang VF*. “Molecular characterization of cryptically circulating rabies virus from ferret badgers, Taiwan.” Emerging Infectious Disease. 2014, 20(5): 790-8. (IF: 7.422; Infectious disease 4/88=4%)
en/hywang/start.txt · Last modified: 2018/11/21 08:19 by 澤大衛 (David Zelený)