國立臺灣大學生態學與演化生物學研究所

Institute of Ecology and Evolutionary Biology
National Taiwan University

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王俊能 教授 (Chun-Neng Wang, Professor)


職稱: 教授

最高學歷: 英國愛丁堡皇家植物園、愛丁堡大學
博士後: 加拿大UBC大學植物園
研究專長: 花發育演化、生殖生物學、高山植物傳粉生態、植物功能基因體、種化適應與親緣地理

E-mail: <LEAFY@@ntu.edu.tw>
研究室: 生命科學館1204室
電話: (02) 33664537
傳真: (02) 23673374
個人網頁:http://homepage.ntu.edu.tw/~leafy/


植物演化發育研究室:研究面向:

實驗室主持人自英國愛丁堡大學皇家植物園取得博士學位,曾經任職加拿大英屬哥倫比亞大學植物園博士後研究員。主要研究領域為花發育演化、授粉生物學、生殖生物學、親緣地理及適應種化。研究專注在花兩側對稱基因功能對花朵形狀之影響、小分子核糖核酸對蘚苔及被子植物的生長發育影響、台灣高山植物花色演化、昆蟲傳粉及植物生殖策略、東亞及台灣特有植物之種化適應,並與日本、大陸鄰近區域學者合作研究群聚生態親緣。

王俊能的實驗室團隊研究,主要專注在花兩側對稱基因對花朵形狀之演化發育功能、小分子核糖核酸對蘚苔(與日本京都大學合作)及開花植物(中研院植微所及生農中心合作)花生長發育的影響、台灣高山植物花色演化、昆蟲傳粉生物學及植物生殖策略(與日本北海道大學合作)、東亞及台灣特有植物之種化適應(與日本筑波植物園、大陸昆明植物所學者合作),並與生演所團隊共同研究台灣植群群聚沿海拔梯度之物種生態棲位與親緣歷史關聯。近期發表主要向學界報導出花對稱性基因CYCLOIDEA (CYC)在苦苣苔科不同類群,基因經常性演化增加出多個複份,從而使新的複份基因受到天擇篩選或人擇栽培馴化,在花芽中產生新的表現形式以調控發育出花兩側-輻射對稱性轉換。藉由遺傳分析,更發現到CYC能控制花開口大小及花冠筒彎曲程度,得以演化出不同花形以適應各種傳粉者訪花。而在台灣高山植物傳粉生態研究上,在採樣近千個原生種後發現高山植物會盡量分化成不同花色以吸引不同傳粉者在花季能專注為期授粉,為學界首次揭開高山植物為何花色多樣的演化成因。而為了瞭解東亞特有的紅豆杉及苦苣苔播遷至台灣的親緣地理歷史,大陸及島嶼族群分化後的基因交流程度及冰河期台灣海峽陸橋環境棲位的模擬資料均顯示,台灣因有高低海拔落差從寒溫帶到亞熱帶的植群氣候帶,提供這些孑遺物種從冰期來避難於台灣,甚至可以成為東亞植物往南至菲律賓,及往東至琉球群島傳播的跳板。這些成果詳見王俊能網頁發表文獻。

實驗室兩大研究主軸,一方面以形態發生學觀念,來探索植物發育之開花調控;另一方向則利用大數據分析台灣植物花色受傳粉者天擇的競爭及適應演化、分子親緣地理、族群遺傳分析,來偵測台灣植物種化及花朵演化機制,下列主題為代表:


**研究方向**:

(1) 花對稱性及花形發育之基因調控 – 以苦苣苔科植物為模式。 【開花植物能演化出多達35萬種出各式各樣花形的變異】,佔領世界各角落,是達爾文畢生認為最不可思議的演化奇蹟。而花的兩側對稱性發育最能促進訪花昆蟲傳粉效率,被認為是開花植物最重要演化趨勢。苦苣苔及蘭科植物花形因與昆蟲共演化而變化多端,深受花卉園藝產業愛好,也成為絕佳研究花發育的模式物種。所以我們以大岩桐、非洲菫及台灣蘭科原種大芋蘭為材料,比較花從兩側對稱突變回輻射對稱的發育轉變,找出控制花對稱性CYCLOIDEA基因及其下游途徑基因,遺傳關聯出基因如何影響花各部分形狀;並透過基因轉殖及上下游調控,確立基因功能及控制花形改變的機制。 圖一、非洲堇兩側對稱花(中央)發育成輻射對稱花(左右)的兩種基因調控模式。取自Hsu et al. (2018) Frontier in Plant Science 9:1008。徐卉汝、何承紋、郭聞喜、辛冠霆博士生及潘昭君博後共同發表。

(2) 植物在地適應演化 – 台灣植物種化機制 【島嶼是物種演化的天然實驗室】。台灣鄰近大陸、琉球與菲律賓,是亞熱帶罕見,冰河孑遺植物避難所,有高達1千多種特有植物。它們何時從冰期陸橋遷徙過來?是否及如何適應海島氣候?而現今海洋造成的地理隔離,是否是台灣植物從鄰近區域種化而來的主因? 我們分析台灣紅豆杉、苦苣苔等植物在東亞族群的遺傳分化,發現第四紀以來基因交流受阻的確促進台灣物種從大陸分化出來,而棲地的氣溫及雨量影響著植物產生適應性演化。由在地環境因子適應分析看來,當氣候變遷如暖化造成雨量不均,將會嚴重影響到台灣植物生存。 圖二、台灣紅豆杉族群遺傳變異(橘色)中有夾雜來自中國的遺傳基因型(綠色)→ 台灣紅豆杉由中國南方紅豆杉(Taxus mairei) 演化過來;菲律賓紅豆杉族群遺傳變異(紅色)中有夾雜來自台灣的遺傳基因型(橘色)→菲律賓紅豆杉由台灣紅豆杉傳播過去。郭浩志博後發表準備中。

(3) 傳粉機制與生殖 – 高中低海拔花色演化與自交異交生殖策略 【花色變化扮演吸引不同傳粉者角色】。台灣為亞熱帶氣候,卻有東亞最高山脈縱橫,海拔高低產生寒溫帶到熱帶森林棲位差異,演化出近四千種開花植物。我們首次完整記錄高山植物是由膜翅目蜂類及雙翅目蠅類為主傳粉,傳粉者多樣性比東亞大陸少但與同是島嶼的日本相當。而花色演化多以亮白及黃色為主,推測視覺上能盡量吸引各類傳粉昆蟲拜訪,但不同種植物會盡量花色差異以避免競爭同類訪花傳粉者。我們也首次記錄到台灣有畫眉科鳥類吸食原生植物長果藤花蜜傳粉的珍貴影像。利用花色反射光譜,我們發現台灣植物以可反射各波長的白色花為多數,可增加吸引不同種類傳粉者訪花。而苦苣苔科長果藤冬季開花,花冠裂片正面反射紅色,吸引在秋末及冬季時以植物花蜜為主食的冠羽畫眉鳥訪花傳粉。另花輻射對稱的苦苣苔,則演化出花謝時花冠筒脫落時,雄蕊碰觸柱頭之自發性自交,來彌補訪花昆蟲不足時之生殖結實。 圖三、長果藤開花與冠羽畫眉訪花行為照片(左)。雄、雌花時期長果藤雄雌蕊相對位置。陳凱修、盧璟誼發表: Chen et al. (2019) Scientific Reports。

(4) 植物營養期與開花期轉換– 日照、開花素與反開花素之交互作用影響成花反轉珠芽 【植物營養/開花時期轉換受到嚴密環境因子調控】。然而苦苣苔科俄式草可在花期終了時,受短日照調控增加反開花素基因(CENTRORADIALIS)表現,花序頂端反轉成營養無性繁殖珠芽。所以計畫利用開花素(FT)與反開花素(CEN)的刺激,將來應用在調整植物如果樹的開花/營養週期轉換,調節產期。 圖四、俄氏草生殖策略兼行開花有性(左)及無性珠芽繁殖(右)。 而花序反轉成珠芽的發育受到光週期縝密調控。

近年研究主題: 1. 大岩桐花對稱性途徑基因與花形發育調控: 除基礎分子發育及切片技術訓練,並利用新一代高速定序平臺(next generation sequencing or NGS),分析背、腹側花瓣有差異性表現的基因(轉錄體 transcriptome + alternative splicing)(與生技所陳仁治老師、中研院植維所涂世隆研究員合作)、並檢測出背、腹花瓣差異表現小分子RNA(microRNA)及降解組(degradome)(與中研院農生中心陳荷明研究員合作),得出背、腹側花瓣各自的發育途徑基因。並成功建立了花瓣原生質體暫時性表達系統(transient expression in petal protoplast)(與生科系林盈仲老師合作),確認有交互作用的上下游基因。 (基因體序列與Virginia Tech維吉尼亞理工大學 Bombarely 教授合作。

2. 大岩桐及俄式草組織培養與轉殖系統建立(與農化系洪傳揚老師合作)、病毒基因靜默系統建立(與中研院農生中心葉信宏研究員合作)

3. 延伸研究小分子RNA 390-TAS3在初始陸生植物地錢中的發育功能,以了解此機制如何適應演化出葉上下表面發育差異,及花對稱性背腹側花瓣差異。

4. 植物親緣及生物地理: 以親緣分析、分子生態方法,進行台灣紅豆杉親緣地理、紅豆杉適應性演化(與昆明植物所及愛丁堡皇家植物園合作);東亞苦苣苔親緣地理(與筑波植物園合作)

5. 花朵傳粉機制與生殖: 台灣野生輻射對稱花苦苣苔、長果藤鳥類傳粉生殖策略;大芋蘭及輻射大芋蘭唇瓣發育演化與自交機制、雙葉蘭唇瓣解剖結構反射UV光吸引傳粉者機制;折柱赤箭自發性絕對自交生殖機制之發育(與台大植科所林讚標教授及科博館李勇毅博士合作)

6. 台灣高中低海拔(反射光譜)花色演化(與昆蟲系楊恩誠老師、北海道大學合作) 。 實驗室目前有一位博士後研究員、三位博士生、四位碩士生及5位大學部學生。畢業學生以出國進修博士(英美丹麥瑞士)、國內研究員(如農業、林業試驗所)、公務員(林務局、國家公園等)為主。

圖五、實驗室2016 Apr 福山植物園retreat。


開設課程

EEB5035演化生物學 Evolutionary Biology
EEB5079親緣地理分析 Phylogeographic analysis
EEB5052植物分子發育及演化 Plant molecular evolution and development
EEB5019維管束植物形態與演化 Morphology and evolution of vascular plants
LS1004普通生物學甲(生科系)、乙(醫學系) General Biology
LS1002服務學習二、三(讓世界聽見玉山的聲音-原聲音樂學校山地學童課輔 Student service
LS1003熱帶植物保育-辜嚴倬雲保種中心 Tropical plant conservation
LS4009發生生物學實驗 Lab of developmental biology
ME4116現代科技與人類文明 Modern technology and civilization
Prog5116專題研究 research topics

代表著作

* denotes corresponding author 

Chen KH, Lu JY, Wang CN* (2019) Specialized pollination system of Aeschynanthus acuminatus (Gesneriaceae) by generalist passerines in sunbird-absent East Asia. Scientific Reports 9:17552.

Leebens-Mack JH, Barker MS, Carpenter EJ,…Wang CN,…, et al. One Thousand Plant Transcriptomes Initiative (2019). One thousand plant transcriptomes and the phylogenomics of green plants. Nature 574: 679–685.

Huang B-H , Nishii K, Wang CN, Moller M (2019). Quantitative assessment of anisocotyly in Haberlea rhodopensis and Ramonda myconi. Edinburgh Journal of Botany 76: 377-391.

Hsin KT, Lu JY, Möller M, Wang CN* (2019) Gene duplication and relaxation from selective constraints of GCYC genes correlated with various floral symmetry patterns in Asiatic Gesneriaceae tribe Trichosporeae. Plos One 14(1): e0210054 hsin2019-gcyc-duplication.pdf

Hsin KT, Wang CN* (2018) Expression shifts of floral symmetry genes correlate to flower actinomorphy in East Asia relic Conandron ramondioides (Gesneriaceae). Botanical studies. 59:24 hsin-wang2018-conandron_cyc.pdf

Hsu HJ, He CW, Kuo WH, Hsin KT, Lu JY, Pan Z, Wang CN* (2018) Genetic analysis of floral symmetry transition in African Violet suggest involvement of trans-acting factor for CYCLOIDEA expression shifts. Frontiers in Plant Science. 9:1008 hsu2018-african_violet.pdf

Kuo WH, Hung YL, Wu HW, Pan ZJ, Hong CY, Wang CN* (2018) Shoot regeneration process and optimization of Agrobacterium mediated transformation in Sinningia speciosa. Plant Cell, Tissue and Organ Culture: 1424-7 kuo2018-sinningia_transformation.pdf

Kuo LY, Ebihara A, Hsu TC, Rouhan G, Huang YM, Wang CN*, Chiou WL, Kato M (2018) Infrageneric Revision of the Fern Genus Deparia (Athyriaceae, Aspleniineae, Polypodiales). Systematic Botany. 43: 645-655 kuo2018-systematic.pdf

Kuo LY, Tang TY, Li FW, Su HJ, Chiou WL, Huang YM, Wang CN* (2018) Organelle Genome Inheritance in Deparia Ferns (Athyriaceae, Aspleniineae, Polypodiales). Frontier in Plant Science. 9: 486 kuo2018-frontier.pdf

Kuo LY, Ebihara A, Kato M, Rouhan G, Ranker TA, Wang CN*, Chiou WL (2018) Morphological characterization of infra-generic lineages in Deparia (Athyriaceae: Polypodiales). Cladistics. 34: 78-92 kuo2018-cladistics.pdf

Hsu HC, Hsu KL, Chan CY, Wang CN, Kuo YF (2018). Quantifying colour and spot characteristics for the ventral petals in Sinningia speciosa. Biosystems Engineering. 167: 40-50 hsu2018-colour_sinningia.pdf

Li YS, Chang CT, Wang CN, Thomas P, Chung JD, Hwang SY (2018) The Contribution of Neutral and Environmentally Dependent Processes in Driving Population and Lineage Divergence in Taiwania (Taiwania cryptomerioides). Frontiers in Plant Science. 9:1148 li2018-taiwania-frontier.pdf

Liu J, Milne RI, Möller M, Zhu GF, Ye LJ, Luo YH, Yang JB, Wambulwa MC, Wang CN, Li DZ, Gao LM (2018) Integrating a comprehensive DNA barcode reference library with a global map of yews (Taxus L.) for forensic identification. Molecular Ecology Resources. 18:1115–1131. liu2018-taxus.pdf

Huang CT, Hsin KT , Wang CN, Liu CT, Kao WY (2018) Phylogenetic analyses of Bradyrhizobium symbionts associated with invasive Crotalaria zanzibarica and its coexisting legumes in Taiwan. Systematic and Applied Microbiology. 41: 619-628 huang2018-crotalaria.pdf

Hsu HC”, Wang CN”, Wang CC, Liang CH, Wang, CC, Kuo YF (2017) Association between petal form variation and CYC2-like genotype in a hybrid Line of Sinningia speciosa. Frontiers in Plant Science. 8:558 ” Co-first author hsu2017-cyc2-sinningia.pdf

Chen YY, Nishii K, A Spada, Wang CN, Sakakibara H, Kojima M, Wright F, MacKenzie K, Möller M (2017) Cytokinin biosynthesis ISOPENTENYLTRANSFERASE genes are differentially expressed during phyllomorph development in the acaulescent Streptocarpus rexii (Gesneriaceae). South African Journal of Botany. 109:96-111. chen2017_sajb_ipt.pdf

Nishii K, Huang BH, Wang CN, Möller M (2017) From shoot to leaf: step-wise shifts in meristem and KNOX1 activity correlate with the evolution of a unifoliate body plan in Gesneriaceae. Development Genes and Evolution. 227:41-60. nishii2017-_knox1.pdf

Lee YI, Chung MC, Kuo HC, Wang CN, Lee YC, Lin CY, Jiang H, Yeh CH (2017) The evolution of genome size and distinct distribution patterns of rDNA in Phalaenopsis (Orchidaceae). Botanical Journal of the Linnean Society. 185:65-80. lee2017-phal_genome.pdf

Su MH, Hsu TH, Wang CN, Lin KH, Chiang MC, Kang RD, Nguyen KM, Nguyen H (2017). Genetic Diversity of a Novel Oil Crop, Camellia brevistyla, Revealed by ISSR DNA Markers. Korean Journal of Horticultural Science and Technology 35: 588-598 su2017-camelia-issr.pdf

Kuo HC, Soisook P, Ho YY, Csorba G, Wang CN, Rossiter S (2017) A taxonomic revision of the Kerivoula hardwickii complex (Chiroptera: Vespertilionidae) with the description of a new species. Acta Chiropterologica. 19: 19-39. kuo2017-chiroptera.pdf

Kuo LY, Ebihara A, Shinohara W, Rouhan G, Wood KR, Wang CN*, Chiou WL (2016 ) Historical biogeography of the fern genus Deparia (Athyriaceae) and its relation with polyploidy. Molecular Phylogenetics and Evolution. 104:123-134. kuo2016_-_biogeography_deparia.pdf

Kuo LY, Chang YH, Glowienka JMO, Amoroso VB, Dong SY, Kao TT, Wang CN*, Chiou WL (2016) A Revised Framework of Dryopteris subg. Nothoperanema (Dryopteridaceae) Inferred from Phylogenetic Evidence, with Descriptions of Two New Sections. Systematic Botany. 41:596-605. kuo2016_-_dryopteris.pdf

Wang CN, Hsu HC, Wang CC, Lee TK, Kuo YF (2015) Quantifying floral shape variation in 3D using microcomputed tomography: a case study of a hybrid line between actinomorphic and zygomorphic flowers. Frontiers in Plant Science. 724: 6 hsu2015-sinningia2d.pdf

Yang CY, Huang YH, Lin CP, Lin YY, Hsu HC, Wang CN, Liu LYD, Shen BN, Lin SS (2015) MicroRNA396-Targeted SHORT VEGETATIVE PHASE Is Required to Repress Flowering and Is Related to the Development of Abnormal Flower Symptoms by the Phyllody Symptoms1 Effector. Plant Physiology. 168: 17021716 yang2015-mi396.pdf

Hsu HC, Chen CY, Lee TK, Weng LK, Yeh DM, Lin TT, Wang CN*, Kuo YF (2015) Quantitative analysis of floral symmetry and tube dilation in an F2 cross of Sinningia speciosa. Scientia Horticulturae. 188: 71-77. hsu2015-sinningia2d.pdf

Nishii K, Ho MJ, Chou YW, Gabotti D, Wang CN*, Spada A, Möller M (2014) GA2 and GA20-oxidase expressions are associated with the meristem position in Streptocarpus rexii (Gesneriaceae). Plant Growth Regulation. 72: 123-140 nishii2014-ga.pdf

Zhang WY, Kuo LY, Li FW, Wang CN*, WL Chiou (2014) The hybrid origin of Adiantum meishanianum (Pteridaceae): A rare and endemic species in Taiwan. Systematic Botany. 39, 1034-1041 zhang2014-adiantum.pdf

Pan ZJ, Chen YY, Du JS, Chen YY, Chung MC, Tsai WC, Wang CN, Chen HH (2014) Flower development of Phalaenopsis orchid involves functionally divergent SEPALLATA-like genes. New Phytologist. 202, 1024-1042 pan2014-sep.pdf

Hsieh YC, Chung JD, Wang CN, Chang CT, Chen CY and Hwang SY (2013) Historical connectivity, contemporary isolation, and local adaptation in a widespread but discontinuously distributed species, Rhododendron oldhamii, endemic to Taiwan. Heredity. 111: 147-156.

Huang CT, Hsieh CF, Wang CN* (2013) Remusatia yunnanensis (Araceae): A Newly Recorded Species in Taiwan. Taiwania. 58:76-79. huang2013-remusatia.pdf

Nishii K, Wang C-N, Spada A, Nagata T, Möller M. (2012) Gibberellin as a suppressor of lateral dominance and inducer of apical growth in the unifoliate Streptocarpus wendlandii. New Zealand Journal of Botany 50: 267-287 nishii2012-ga_nzjb.pdf

Nishii K, Nagata T, Wang C-N, Möller, M.(2012) Light as environmental regulator for germination and macrocotyledon development in Streptocarpus rexii (Gesneriaceae). South African Journal of Botany 81: 50-60. nishii_et_al_2012_sajb.pdf

Chen CW, Kuo, LY, Wang C-N, Chiou WL(2012) Development of PCR primer sets for intron 1 of the low-copy gene leafy in Davalliaceae. American Journal of Botany 99: e223-225.

Chou YW, Thomas PI, Ge XJ, Lepage BA, Wang CN*(2011) Refugia and phylogeography of Taiwania in East Asia. Journal of Biogeography 38: 1992-2005 chou2011-taiwania.pdf

Kuo LY, Li FW, Chiou WL, Wang CN* (2011) First insights into fern matK phylogeny. Molecular Phylogenetics and Evolution 59: 556-566 kuo2011-fern-matk.pdf

Nishii K, Möller M, Kidner C, Spada A, Mantegazza R, Wang C-N, Nagata T(2010) A complex case of simple leaves: Indeterminate leaves co-express ARP and KNOX1 genes. Development Genes and Evolution 220: 25-40 nishii2010.pdf

Li FW, Kuo LY, Huang YM, Chiou WL, Wang CN* (2010) Tissue-direct PCR, a rapid and extraction-free method for barcoding of ferns. Molecular Ecology Resources 10: 92-95 li2010-tissuepcr-mer.pdf

Nishii K, Nagata T, Wang CN* (2009) High morphological plasticity in Gesneriaceae meristems: Reversions in vegetative and floral development. Trends in Developmental Biology 4: 33-40 nishii-wang2008-rev.pdf

Li FW, Tan BC, Buchbender V, Moran RC, Rouhan G, Wang CN* , Quandt D(2009) Identifying a mysterious aquatic fern gametophyte. Plant Systematics and Evolution 281: 77-86. li2009-mysterious_fern-pse.pdf

Lin YH, Hwang SY, Hsu PY, Chiang YC, Huang CL, Wang CN, Lin TP.(2008) Molecular population genetics and gene expression analysis of duplicated CBF genes of Arabidopsis thaliana. BMC Plant Biology 8: 111.

Wang CN*, Chen YJ, Chang YC, Wu CH(2008) A step-by-step optimization guide for applying tissue specific RNA in-situ hybridization to non-model plant species. Taiwania 53: 383-393 wang-ish-taiwania.2008.53.4.383.pdf

Wang CN*, Möller M, Cronk QCB (2004a) Population genetic structure of Titanotrichum oldhamii (Gesneriaceae), a subtropical bulbiliferous plant with mixed sexual and asexual reproduction. Annals of Botany 93: 201-209 titano-aob-rapd.pdf

Wang CN*, Möller M, Cronk QCB (2004b) Aspects of sexual failure in the reproductive process of a rare bulbiliferous plant, Titanotrichum oldhamii (Gesneriaceae), in subtropical Asia. Sexual Plant Reproduction 17: 23-31 sexplant-17-23-31.pdf

Wang CN*, Möller M, Cronk QCB (2004c) Phylogenetic position of Titanotrichum oldhamii (Gesneriaceae) inferred from four different gene regions. Systematic Botany 29: 407-418 wang-sysbot.pdf

Wang CN*, Möller M, Cronk QCB(2004d) Altered expression of GFLO, the Gesneriaceae homologue of FLORICAULA/LEAFY, is associted with the transition to bulbil formation in Titanotrichum oldhamii. Development Genes and Evolution 214: 122-127 gflo-dge.pdf

Wang CN*, Cronk QCB(2003) Meristem fate and bulbil formation in Titanotrichum (Gesneriaceae). American Journal of Botany 90: 1696-1707 ajb-1696-1707.pdf

zh-tw/cnwang/start.txt · Last modified: 2019/11/02 22:58 by 王俊能 (Chun-Neng Wang)